Isolation and characterization of lupeol from methanolic extract of leaves of andrographis echioides

In the present study was to isolate and characterize the lupeol from the methanolic extract of leaves of Andrographis echioides. The isolation was done using column chromatography using gradient elution with different mobile phases. The isolated compound was subjected to spectral analysis. Structure elucidation was carried out on basis of spectral analysis. Purity was checked by high performance thin-layer chromatography (HPTLC) and high performance liquid chromatography (HPLC) technique. The infra-red spectra showed specific absorption bands for lupeol viz. 3432.72 cm-1 (Hydrogen bonded OH Stretch), 2943.72 cm-1 and 2356.85 cm-1 (C-H Stretch in CH2 and CH3), 2104.73 cm-1 (C≡C Stretch), 1661.92 cm-1 (C=C Symmetric Stretch), 1562.63 (C=C Asymmetric stretch), 1416.23 cm-1 (C-H deformation in CH2 and CH3), 1035.38 cm-1 (C-O Stretch of secondary alcohol), 887.84 cm-1 (=C-H bending exocyclic CH2). Structural elucidation of lupeol was done by spectrum analysis such as 13C and 1H depth nuclear magnetic resources. Mass spectra of lupeol showed a parent molecular ion [M+] peak at m/z 426 which corresponds to molecular formula C30H50O. From the spectral characteristics, the isolated compound from the methanolic extract of leaves of Andrographis echioides was confirmed to be lupeol.