The antifungal activity of Benzene, Diethyl ether, Distilled water extract and Ethyl acetate extracts of selected medicinal plants such as A. indica A. Juss., C. roseus (L.) G. Don, J.adhatoda L., O. tenuiflorum L. and V. negundo L. against the P. palmivora (Butl.) revealed that V. negundo L. plant extracts exhibited maximum antifungal activity. The antifungal activity of the V. negundo L. ethyl acetate extract was found to be comparable to the standard fungicides Copper oxychloride and Bordeaux mixture tested. The results indicated that the V. negundo L. plant extracts are good antifungal agents. Totally 29 prominent peaks were obtained in the mass spectrum of V. negundo L. plant extract. The major phytocompounds were 2-Butanol, 4-(2,2-dimethyl-6-methylenecyclohexylidene)- (18.115 %) and 4,8,13-Cyclotetradecatriene-1,3-diol,1,5,9-trimethyl-12-1-met (15.226 %). Present study showed that V. negundo L. plant extracts are potential biocontrol agents for root rot disease in Papaya plant.