Alterations in ascorbic acid contents after chronic exposure to zinc, copper, cadmium and lead for 10 and 20 days, were observed in whole soft body tissue of three experimental snail species, Bellamyabengalensis, Mellanoidestuberculata and Lymnaeaacuminate collected from Dedargaon reservoir of Dhule district of Maharashtra, India. The results indicates that ascorbic acid contents of three experimental snail species were decreased as compared to those of control snails. Among all treated heavy metals, Cd caused more decrease as compared to Zn, Cu and Pb in three snail species. The ascorbic acid decreased was more after 20 days as compared to 10 days of exposure. A maximum ascorbic acid contents were observed in whole soft tissue of Lymnaeaacuminata in response to accumulated levels of Zn, Cu and Pb while Mellanoidestuberculata for Cdas compare to studied snail species. Therefore it is concluded that, snail species Lymnaeacuminata is the sentinel organism for biochemical study of heavy metals Zn, Cu and Pb, and Mellanoidestuberculata for Cd in freshwater ecosystem.
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