A present investigation designed to compare the haematological and biochemical parameters in healthy and infected Clarias batrachus. Haematological and serum biochemical studies on fishes have assumed to be greater significance due to the increasing emphasis and greater awareness of the pollution of natural aquatic bodies. Changes in haematological and biochemical parameters depend on the fish species, age, sex and health condition. These parameters also changes due to environmental stress and fish size. The aim of the present study was to investigate the reference ranges for haematological and serum biochemical values of Clarias batrachus. A total of 10 mature fish were collected. Blood samples were then taken from the caudal vein of fishes. Haematological parameters such as haemoglobin (Hb), total leukocyte count (TLC), packed cell volume (PCV), four parameters namely RBC, Hb (p<0.01) and WBC (p<0.05) revealed significant difference between healthy and infected fish. The findings would be helpful to establish a baseline value and to draw conclusive remarks against the health status of Clarias batrachus, showing deviation from the normal parameters.