Background: Yoga enhanced our own virtual power, our mental and physical power. According to Gherand samhita, mudra is a posture, which enhanced our alertness, maintains the state of wakefulness. The Gyan mudra considered one of the most important mudras. It has been practiced in meditation for thousands of years and continues to bring peace, calm and spiritual progress. Known as the “Mudra of knowledge”. It stimulates wisdom and knowledge.Ptesent study aimsto describe the physiology of Gyan Mudra by understanding its anatomical pathway and analytical survey study.For this purposewe select 50 patients age group 15 to 25yrs student male and female both, after 6 months study result is in our side. On the basis of this survey physiology of Gyan mudraand its association with modern concept will be understand.