A total of 139 urine samples including E.coli UTI and UTI suspected samples were collected from Coimbatore and surrounding districts of Tamil Nadu, India. The samples were assayed by various molecular biology tools like RAPD, ERIC-PCR, REP-PCR and 16SrRNA identification and compared with a standard strain MTCC 326. The RAPD, ERIC and REP profiles were done for the samples. The results showed sensitivity and accuracy in the molecular detection and estimation of E.coli from clinical samples. The methods were validated and optimized against conventional phenotypic identification methods due to high efficiency, accuracy and reproducibility within limited time duration.
Impact Factor
2022: 6.012
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