Natural products have played a very important role in health care and prevention of diseases. The investigation was carried out to determine the chemical constituents of the ester and extract of Stevia rebaudiana using GC-MS.GC-MS analysis of the ester and ethanol extract led to identification of 13 and 10 compounds The compounds found in ester extract are Acetophenone, 4-hydroxy-3, 5-dimethoxy, oxime, bis-TMS, 7-Octadecyne, Butyl tridecyl ether, Succinic acid, cyclohexylmethyl 2-pentyl ester, Cyclobutanecarboxamide, N-decyl-N-methyl, 1,2-Benzene dicarboxylic acid,dibutyl ester, Decane, Undecane, 1-Hexanamine, N-isopropylidene, 9-Octadecenamide. The compounds were identified by comparing their Retention time and covate indexes with that of literature and by interpretation of mass spectra. The compounds found in ethanol extract are Benzene, 1,3-dimethyl, 1-Hexene, 2,4,6-tris-trimethylsilyl, Methyl farnesoate, Hexadecanoic acid methyl ester, Pentadecanoic acid methyl ester, Nonyl pentyl ether, 1,2-Benzene dicarboxylic acid,dibutyl ester, Tricosanol, Heptacosane, Nonacosane and Octacosane.
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