Background: This research investigates a perception-based examination of the supply chain within the Indian seafood sector, aiming to shed light on the intricate dynamics and challenges that characterize this vital industry. The Indian seafood sector holds significant importance both within the domestic and international contexts, with the country being one of the largest global exporters of seafood products. By adopting a perception-based approach, this study aims to uncover the views, experiences, and assessments of various stakeholders, including fishermen, seafood processors, exporters, regulators, and consumers, concerning the Indian seafood supply chain. Method: A survey approach was employed, consisting of approximately 150 key who were asked Likert scale questions, providing a comprehensive view of participants' perceptions and opinions regarding the supply chain. Findings: The study revealed that a majority of participants are familiar with the seafood supply chain processes, although opinions regarding its efficiency are divided. Importantly, most respondents expressed satisfaction with the transparency of information flow within the supply chain.