An important component of orthodontic appliance is the orthodontic bracket. When sliding mechanics are applied, it is either the bracket or the arch wire that slides through the bracket slot. Whenever two different surfaces meet, the resultant effect was friction and overcoming this friction is crucial for a successful treatment outcome. Aim & Objective: The aim and objective of the present study was to analyse the surface roughness of stainless-steel brackets, as received from the manufacturer and from the oral cavity of patients post orthodontic treatment with bicuspid extraction. Material and Methods: Surface roughness was qualitatively analyzed of retrieved and as received orthodontic brackets at the slot and floor, using a three-dimensional non-contact surface profilometer. Results: The surface roughness of the retrieved brackets was greater when compared to the as received brackets. This indicates that there were differences in the surface roughness across the as received and retrieved brackets. Conclusion: Orthodontic treatment brought about a significant increase in the surface roughness and COF of brackets. However, there was no significant difference in the surface roughness at the mesial and distal slots of as received and retrieved brackets.