Vishwamitri River water sample has been analyzed for the parameter of pH, BOD, DO, COD, Phosphate, Conductivity and Total Coliform Organisms. River water samples were seasonally collected at Sama-Savil Road, Sayajibaug Road, Vinoba Bhave Road, Shankheshwar Parshwanath Marg and Vishvamitri Road locations. Chemical and Microbiological Test results does not classified the Vishwamitri river water in Class-A,B or C Category, hence it is recommended water treatment and disinfection process of river water before its use for drinking purpose.