Medicinal plants have been used for centuries and have become beneficial therapeutic medicines worldwide because of their potential health benefits. Some of their metabolites have been successfully used directly in the treatment and prevention of infectious diseases and cancer, or indirectly by stimulating the immune system. Most immunological studies necessitate evaluation of both humoral and CMI responses. Lymphocyte proliferation assays are widely used to assess the cell-mediated immunity.
Lymphocyte proliferation assay were carried out using the peripheral blood mononuclear cells (PBMC) with mitogen induction by phytohemagglutinin-L (PHA-L) and proliferation in cell cultures and whole blood were monitored using colorimetric (MTT) assay. Free radical scavenging capacity as a measure of antioxidant capacity was determined by DPPH inhibition method.
In vitro human peripheral blood mononuclear cells (PBMC) treated with methanol extract of Evolvulus alsinoids (62.5 to 500 μg/mL) showed lymphoproliferation at the concentration of 250 µg/ml, which were significantly higher proliferation when compared with media control (p < 0.05). The extract exhibited greater than 75% scavenging activity at 1000 μg/mL compared with the ethanol vehicle control.
The whole plant Evolvulus alsinoids extracts showed strongest effects on antioxidant activity compared to control.
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