Evaluation of external apical root resorption using cbct and its association with il-6 snp (rs 1800796) as a risk factor after orthodontic treatment in local population

Aims and Objectives: To evaluate the relationship of IL-6 (rs 1800796) GC with external apical root resorption using DNA sequencing and to accurately measure the extent of external apical root resorption using cone beam computed tomography. Methods: DNA samples of 30 subjects with non-syndromic tooth agenesis collected from the department were used for the study. CBCT of maxillary central incisor before and after treatment of 30 patients were also recorded. The extracted DNA samples were subjected to Polymerase chain reaction in which amplification of the selected gene segments was carried out; later these amplified products were subjected to DNA sequencing. Results were documented in the form of electropherograms. Results: • The mean amount of EARR seen across all 30 subjects was 0.45mm which was statistically significant (p<0.001). • EARR of >1mm was seen is 6 subjects of which 2 subjects showed a positive expression of IL-6 SNP GC (22.2 %, with p value=0.59) which was not statically significant. • EARR of ≤1mm was seen is 11 subjects of which 5 subjects showed a positive expression of IL-6 SNP GC (45.4%, with p value=0.59) which was not statically significant. Conclusion 1. The mean amount of EARR seen across all 30 subjects was 0.45mm which was statistically significant. The association of gender with EARR showed a higher predilection towards females than males. 2. EARR of >1mm was seen is 6 subjects of which 2 subjects showed a positive expression of IL-6 SNP GC which was not statically significant. 3. EARR of ≤1mm was seen is 11 subjects of which 5 subjects showed a positive expression of IL-6 SNP GC. Hence showing only a mild correlation of IL-6 SNP GC as a risk factor following orthodontic treatment which was not statistically significant.