This study aims to evaluate digestibility in feed ruminants based on unsaturated fatty acids in vitro. This research used cow rumen fluid, forage substrates and concentrates with a ratio of 60:40. The measured variables were digestibility values including: dry matter digestibility, and organic matter in vitro. The experimental design used was Completely Randomized Design consisting of 4 treatments consisting of: (R0) feed without soybean oil, (R1): feed with 2.5% soybean oil; (R2): feed with 5% soybean oil; (R3): feed with soybean oil 7.5% in 100% dry matter (BK). While for experiment using cakalang fish oil was consisted of (R0) feed without fish oil, (R1): feed with fish oil 2.5%; (R2): feed with fish oil 5%; (R3): feed with fish oil 7.5% in 100% dry matter (BK). Each treatment was performed four replications. The results showed that both soybean oil and cakalang fish oil had significant effect (P<0.05) on the digestibility of dry matter and organic matter. The conclusion in this research is as many as 2.5% of both soybean oil and fish oil could be used in ruminant diets because it is able to maintain good digestibility of dry matter and organic matter. Digestibility value of 2.5% use of soybean oil and cakalang fish oil gives the digestibility value such as without adding oil.