People can become unwell from airborne microorganisms, and Cassia auriculata is used to treat many chronic ailments. Comparing the airborne microbe population before and after employing a spray was the study's main goal. Several extracts, including ethyl acetate, butyl alcohol, acetone, methanol, and distilled water, were tested for antimicrobial activity using the agar well diffusion method on bacteria like Escherichia coli, Staphylococcus sp., Pseudomonas sp., and Proteus sp. and fungi like Aspergillus sp. and Candida sp. The phytochemicals and functional groups were observed as GC-MS and FT-IR methanol flower extract revealed the functional group of the leaf extracts respectively. Thin layer chromatography and the Rf values of bioactive compounds were found. Escherichia coli (23 mm) and candida sp. (25mm) were detected in the floral extract of Cassia auriculata. We discovered MIC varies from 50µl to 400µl of 0.92 to 0.52 OD value and MBC ranges from 50µl to 400µl of 53 to NIL (CFU). In this test, the phytochemicals were found to exhibit antioxidant, anti-inflammatory, and anti-diabetic characteristics that were reflected in the methanol solvent. Using the settle plate method, the air contamination in a dangerous area was evaluated. As a result, we concluded that Cassia auriculata flower extract has the potential to be more successful in antimicrobial activity, controlling the air-borne pathogen and it contains various biological components.
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