Consequence of the chemical mutagen EMS (Ethyl Methane Sulphonate) has been studied on the vegetable crop Spinacia oleracea L. Mutagenic treatments were given in the form of 6 Hrs pre-soaking in EMS, after pre-soaking them in distilled water for 12 hrs. Various physiological parameters like proteins, carbohydrates were evaluated, along with chlorophyll content analysis. The level of amino acid proline was also assessed. It was found that treatment found to have significant inhibitory effect on seed germination. Strong reduction in parameters like proteins and chlorophyll was recorded. In the plants treated with EMS there was significant increase in the level of amino acid proline which is a stress induced amino acid. There was a considerable increase in amount of total carbohydtrates in plants with EMS treatments specially up to 50 mM dose of EMS. EMS found to effectively to induce changes in important physiological parameters.
Impact Factor
2022: 6.012
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