Electrophoretic studies on the muscle proteins of four octopuses represented in the trawl net by-catches off visakhapatnam, east coast of india

The comparative electrophoretic patterns of muscle protein in the four octopus species, Octopus aegina, O. membranaceus, O. dollfusi and Cistopus indicus were reported in the present study. The stnadard molecular weights of protein were found to be 232, 212, 140, 116-128, 94, 76, 67, 30, 20, 14, 6.2 and 3.5 KDa corresponds to catalase, myosin, lactate dehydrogenase, β-galactosidase, phoshorelase b, transferrin, albumin, carbonic anhydrase, tripsin inhibitor, α-lactalbumin, aprotinin, insulin B-chain. The molecular weights of protein bands were found to be 232, 126, 67, 20, 14, 6.2, and 3.5 KDa in O.aegina; 212, 122, 67, 20, 14, 6.2, 3.5 in O. membranaceu; 232, 116, 76, 30, 20, 14, 6.2, 3.5 Kda in O. dollfusi; 140, 94, 20, 14, 6.2, and 3.5 KDa in C. indicus. The cluster analysis showed that the species C. indicus was differing from other three species O.aegina, O. dollfusi and O. membranaceus. Within the three species of octopuses, O.aegina and O.membranaceus closely related than O. dollfusi.