The present study is aimed to investigate the effect of Zinc on protease, amylase and lipase activity in the intestinal bulb and intestine of freshwater fish Gonoproktopterus kolus (Sykes) after acute exposure. During experiment fishes were exposed to predetermined LC0 (2.430 ppm) and LC50 (4.860 ppm) concentrations of the Zinc sulphate for 96 hours. The effect of heavy metal showed decrease in protease, amylase and lipase activity in the intestine of fish. The significant alternations showed toxic effect of heavy metal at enzymatic level. The results are discussed with available literature.