Effect of variable doses of cisplatin on microanatomy of kidney in albino rats

Cisplatin is an inorganic metal complex, and its cytotoxic properties were discovered serendipitously. Cisplatin interferes with DNA replication and kills the fastest proliferating cells. It causes apoptosis in view of the fact that the growth of normal cells is also affected. These toxic effects alter the metabolic function of certain tissues and organs. 60 albino rats weighing on an average 100 grams were taken. The animals were divided into three groups. Group A animals received no drug. Group B were injected with 1.3mg/ m2 of cisplatin by intraperitoneal route and group C 2.5mg/2. The process of drug administration was continued for 12 weeks. The animals were sacrificed in five sittings, after interval of 1,3,6,9 and 12 weeks after drug administration. At the termination of experiments the kidneys were fixed and stained with H&E. In kidney no apparent gross change was found in any of the groups. Histologically it was found that renal corpuscles were affected in high dose group from 3rdweek onwards. Odeama of bowmans space and tubules was seen in high dose group from 6th week onwards .Necrosis of tubular epithelium was profound in high dose group at 12th week. Therefore careful kidney function monitoring is required in patients who are on cisplatin therapy.