The effect of sesame oil as triglycerides on staphylococcal fatty acid modifying enzyme (fame) and abscess formation and compare its effect with true triglycerides

Staphylococcus aureus(S. aureus) subsp. anaerobius was isolated from sheep abscess for extraction of FAME by culturing it on trypticase soy broth medium.
Eight sheep, divided into four groups of two animals each, were inoculated subcutaneously (S/C) with different protocols and doses. Inocula included: S. aureussubsp. anaerobius plus sesame oil; orplus sesame oilandFAME; or plus triglyceride; orplustriglyceride andFAME. The control groups (two groups of two animals each) were inoculated with the enzyme FAME, sesame oil, triglycerides and S. aureussubsp. anaerobius.
Triglycerides (true or sesame oil) were found to inhibit the formation of abscess when injected with abscess-producer Staphylococci (S. aureus subsp. anaerobius), but when FAME was added and when the concentration of the organism was increased, abscess was formed in case of sesame oil, but abscess was small and its nature was changed, whilesites injected with S. aureussubsp. anaerobius plus triglycerides showed slight inflammatory reaction.
Control group, injection of S. aureussubspanaerobius alone grossly showed abscess formation, while the inoculation of FAME, sesame oil and triglycerides produced no lesions at the inoculation sites. All injected sites were confirmed histopathologically.