Effect of physico-chemical factors on phytofabrication of silver nanoparticles by rhizome extract of maranta arundinacea l. its anti-bacterial efficacy

Background: Green nanotechnology is gaining importance due to the elimination of harmful chemicals and provides a safe, environmental friendly and economic method for synthesis of nanoparticles.
Methods: In the present investigation rhizome extract of Maranta arundinacea L. was used for synthesis of silver nanoparticles which was confirmed by changing the colour of reaction mixture from colourless to brown. Synthesized nanoparticles were characterized by UV-vis, FTIR, XRD, EDX, AFM, HR-TEM and testing the antibacterial activity against E. coli and S. aureus.
Results: Silver nanoparticles shows characteristic UV-Vis absorption peak at 410 nm. The effect of physico-chemical parameters – pH, reaction temperature, rhizome extract and silver nitrate concentration were studied and optimized nanoparticles of diverse sizes. Synthesized silver nanoparticles were further characterized by FTIR, EDX, AFM and HR-TEM. The size of silver nanoparticles ranges from 30 to 70 nm and are spherical in shape. Synthesized silver nanoparticles were crystalline in nature. Silver nanoparticles were highly toxic to E. coli than the S. aureus.
Conclusion: Physico-chemical conditions of reaction mixture have been standardized to obtain sharp intense absorption peaks which indicate that formation of small sized monodispered silver nanoparticles. Silver nanoparticles show good antibacterial activity against E. coli and S. aureus.