Microorganisms were isolated from fruit waste disposable area soil samples of Pazhamuthir Nilayam, Coimbatore, Tamil Nadu and screened in modified Czapek- Dox media with citrus pectin as sole carbon source for its pectinolytic activity. Among the isolates, only four fungal strains showed hydrolyzing zone, and was found predominant in Paecilomyces variotii with a clearance zone of 36 mm out of colony diameter of 57 mm was selected and hence, Paecilomyces variotii was selected as a candidate for the study. The effects of the various carbon and nitrogen sources on the pectinase activity by Paecilomyces variotii was carried out in surface culture fermentation. The commercial carbon and nitrogen sources included dextrose, maltose, cellulose, ammonium sulphate, potassium nitrate and urea respectively. The results of the investigation revealed that the maximum pectinase production of 9.66±0.17 Umg-1 protein and of 9.62±0.11 Umg-1 protein was obtained at both intracellular and extracellular level respectively were observed in 1% and 2% maltose as carbon sources. Among the nitrogen sources the maximum pectinase activity of 9.73±0.10Umg-1 protein and 7.44±0.12 Umg-1 protein was observed in 3% ammonium sulphate at both intracellular and extracellular level respectively.
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