Pioglitazone and glimepiride are the anti-diabetic agents used in the treatment of type-2 diabetes. There are very few methods to analyze Pioglitazone and glimepiride individually or in combination form. The present work describes a Reverse Phase High Performance Liquid Chromatography for simultaneous estimation of pioglitazone and glimepiride in bulk and pharmaceutical dosage form on RP C-18 Column (15 cm x 4.6 mm, 5 μm) using methanol and water (80:20 v/v) as mobile phase at a flow rate of 1.0 ml/min and the detection wavelength was 251 nm. The retention time of pioglitazone and glimepiride was found to be 1.9 min. and 6.6 min. respectively. Linearity was observed in the concentration range of 15-75 μg/mL for pioglitazone and 2-10 μg/ml for glimepiride. Percent recoveries obtained for pioglitazone and glimepiride were 99.62 and 101 respectively.
Impact Factor
2022: 6.012
This work is licensed under a