Introduction: The application of the surgical safety checklist is of vital importance to improve the quality of surgical care, and likewise safeguard the safety of patients, minimizing the various most common avoidable adverse events that can endanger the life and well-being of patients, during surgery. Objective: To determine compliance with the safe surgery checklist in the nursing staff of the operating room service of the General Hospital. Methodology: Quantitative, cross-sectional, descriptive and observational study. Where the study population was the nursing staff working in the operating room service, through the use of the observation guide and application of a questionnaire. Results: A total of 18 nursing staff were taken into account. Compliance with the LVCS in the general hospital by the nursing professional was 5.6% excellently and 33.3% did not comply. Thelevel of knowledge ofthe nursing staff was found to be that only 16.7% have high knowledge and 5.6% have low knowledge. According to the three phases of the safe surgery checklist: at the entrance it was fulfilled at least with 27.8% and did not comply with 27.8%; in the pause it was fulfilled at least with 66.7% and in the exit it is significantly met with 38.9%. The relationship between knowledge and compliance with the safe surgery checklist by nursing staff at Hospital General de Acapulco was not statistically significant. Conclusion: Most nursing staff in the operating room service of Acapulco General Hospital do not comply with the safe surgery checklist. Knowledge about the safe surgery checklist among nursing staff in the Operating Rooms of the General Hospital was at a medium level.