Mitotic figures are one of the major criteria to assess dysplasia. Various methods to illustrate mitotic figures have been developed over the years including microscopy, morphometry, flow cytometry, nucleotide radiolabelling and immunohistochemistry. But these methods are not cost effective and less feasible for routine use. Selective stains such as crystal violet, giemsa, toluidine blue, feulgen have been used for staining mitotic figures in tissues. The present study aims to evaluate effectiveness of various stains for studying mitotic figures and comparison of mitotic figures in oral epithelial dysplasia and oral squamous cell carcinoma. Material & methods: The study sample includes sections from tissues embedded in paraffin blocks diagnosed as oral epithelial dysplasia and oral squamous cell carcinoma. These sections will be stained with various stains and the mitotic figures will be assessed. Results: Mitotic figures were enhanced with feulgen stain. Although no statistically significant results were obtained on comparing the study groups. Conclusion: The study will be further continued with greater sample size for quantification of mitotic figures with H & E and Feulgen stain.
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2022: 6.012
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