Effect of ethanol and aqueous extracts of different parts of medicinal plant Euphorbia hirta L. were examined using agar disc diffusion method against some pathogens such as Escherichia coli and Staphylococcus aureus. Leaf, bud and stem were extracted using ethanol and aqueous as solvent. In case of E. coli, maximum in vitro inhibition was scored in bud extract using aqueous solvent which offered inhibition zone of 20 mm and zone of inhibition area of 471.00 mm². In case of S. aureus, both leaf and bud extracts in ethanol and bud and stem extracts in aqueous solvent which offered same inhibition zone of 15 mm and zone of inhibition area of 294.38 mm² A significant inhibition was also found in other extracts of Euphorbia hirta against E. coli and S. aureus.