The aim of this study was to assess the relationship between Diabetes mellitus and Glaucoma by checking the association between blood sugar levels (BSL) and corresponding intraocular pressure (IOP) in diabetic and non-diabetic patients. Eighty-two non-diabetic and Seventy-one diabetic subjects were part of the study. The subjects underwent complete ocular examination. IOP using applanation tonometry, at Fasting and postprandial was recorded. BSL at Fasting and Postprandial was measured by Glucose oxidase/Peroxidase method. Postprandial IOP was significantly higher than baseline IOP in diabetic (18.01 ± 3.55 versus 15.07 ± 3.23 mmHg; p < 0.001) and non-diabetic patients (14.58 ± 3.31 versus 12.06 ± 2.50 mmHg; p < 0.001). Postprandial BSL were significantly higher than baseline measurements in both diabetic (mean increase of 79.18 mg/dL; p < 0.001) and non-diabetic patients (mean increase of 20.48 mg/dL; p < 0.001). Correlative analysis showed a very statistically significant association between post-prandial BSL and post-prandial IOP in diabetic subjects with Pearson’s coefficient at 0.3728 (p<0.0001). For non-diabetic patients. Correlative analysis showed a lesser significant correlation with Pearson’s coefficient at 0.1739 (p<0.05). We concluded that there is a significant association between BSL and IOP variation, especially in diabetic patients.