Urinary tract infections (UTI) refers to an inflammatory response of urothelium towards bacterial invasion which is associated with bacteruria and pyuria1. Aim of the present study is to compare bacteriological profile among diabetic and non-diabetic patients suffering from symptomatic urinary tract infection and to know the antibiotic sensitivity pattern. Materials and Method: Urine samples (midstream sample) was collected by clean catch technique in a sterile leak proof container from both diabetic and non-diabetic patients and after centrifugation, supernant was discarded and sediments were used for gram staining and culture . After overnight incubation at 37⁰ C, growth was noted and identification was done using standard procedures. Antibiotic sensitivity was done on Muller Hinton agar using Kirby baeur disk diffusion method and zone of inhibition were interpreted as per CLSI guidelines. Result: A total of 300 patients were included in the study, out of which 170 (57%) were diabetic while 130(43.3%) were non diabetic. Samples were taken from 300 patients from which 256(85.33%) were culture positive while 44(15%) were culture negative. Among both diabetic and non-diabetic patients females were most commonly suffering from UTI. Among diabetic patients female 94(69.62%) were more commonly suffering from UTI as compared to males 41(30.4%). Similarly females 75(62%) are more commonly affected by UTI among non-diabetic group as compared to males 46(38%). Urine culture from both diabetic and non-diabetic culture showed gram negative bacteria were more commonly responsible for UTI as compared to gram positive cocci. Gram negative bacteria accounted to 87.5% of the infections while 12.5% isolated were gram positive cocci. Most common organism responsible for UTI in both the group was E. coli 112(44%), followed by Klebsiella species 70 (27.34%), Proteus species (9%) while among gram positive cocci, Enterococcus species 16(7.81%) were most common followed by Staphylococcus aureus 10(4%) and coagulase negative Staphylococcus aureus 3(1.2%). Among diabetic patients most common age group affected with UTI was 51-60 years 75(55.5%) followed by 41-50 years 35(26%), 61-70years 15(11.11%) and 31-40 years10 (7.4%) while in non-diabetic patients most common age group affected by UTI was 21-30 years 40(33.05%) followed by 31-40years 30(25%), 41-50 years 22(18.2%). No significant difference in antibiotic susceptibility pattern was seen among both the groups. In gram negative bacteria, maximum sensitivity was seen for imipenem 184(82%), Amikacin 168(75%), Piperacillin-tazobactum 168(75%), Ceftriaxone 139(62%) while least sensitive were Ciprofloxacin 56(25%) and Cotrimoxazole 34 (15%). Among gram positive isolates all were sensitive to Vancomycin and Linezolid. Least sensitivity was seen with Ciprofloxacin 8(25%), Erythromycin 24(75%), Cotrimoxazole 20 (62%).
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2022: 6.012
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