Ralstonia solanacearum a proteobacterium, is responsible for causing wilting in a wide host range of plants specially to solanaceae family including potato, tomato, etc. R. solanacearum has many proteins that act as virulence factors and cause wilting by colonizing the xylem vessels functioning as cellulase enzyme thereby blocking the water transport and finally facilitating wilting. To control the crop loss caused by the organism, an identification kit for its early detection is being manufactured. The kit will work on the principle of antigen-antibody interaction where the causing agent i.e. the Egl protein will be taken as antigen and monoclonal antibodies will be produced against it. Therefore, the Egl gene is cloned here which would be further expressed in order to produce a bulk amount of protein for antibody preparation.
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