India has emerged as the fastest growing major economy in the world. The Indian economy is the sixth-largest in the world measured by nominal GDP and the third-largest by purchasing power parity (PPP). The country is classified as a newly industrialized country, and one of the G-20 major economies. The long-term growth prospective of the Indian economy is positive due to its young population, corresponding low dependency ratio, healthy savings and investment rates, and increasing integration into the global economy. Make in India is the best initiative of government of India which provides such a suitable platform for investment and ease of doing business. This Make in India campaign guides the foreign investors, prompt response, assistance to foreign investors and provide relevant information and proactive approach. Biotechnology is the most promising sector recognized under make in India. The Indian biotechnology sector is one of the fastest growing knowledge-based sectors in India and is expected to play a key role in shaping India's rapidly developing economy. With numerous comparative advantages in terms of research and development (R&D) facilities, knowledge, skills, and cost effectiveness, the biotechnology industry in India has immense potential to emerge as a global key player. India is among the 12 biotech destinations in the world and ranks third in the Asia - pacific. This sector in India, which is currently growing at 20%, is expected to go up to USD 11.6 Billion by end of 2017. The focus is on making the Indian biotechnology sector reach USD 100 billion by 2025. This communication covers overview of the Make in India campaign, sectors covered, impact of make in India in biotechnology sector, Initiatives taken by government of India, FDI Policy, challenges, opportunities and foreign investment in Indian manufacturing. The present study is based on secondary data which has been extracted from the various sources like research articles, publications from Department of industrial policy and promotion, DBT, GoI, DST, GoI, union budget reports, various bulletins of RBI and authenticated websites.