Nanoscience is developing very rapidly in all fields of science and so in the field of pharmacy, where it is actually very demanding, as the global problem of multi drug resistance is arising all over. Therefore to overcome this issue nano science is proving beneficial, by the production of nanoparticles from various organic and non organic materials. Here we have synthesized silver nanoparticles from the peels of raw mango. After keeping the material for 24 hours at stationary condition change in colour was observed this indicated the production of silver nanoparticles. Further characterization using UV spectroscopy, XRD, FTIR, SEM and TEM of the solution was done which confirmed the synthesis of silver nanoparticles. Later antibacterial assay was followed to determine the antibacterial potential of the solution against the bacteria E. coli. Maximum zone of inhibition of 21mm is observed in the concentration of 3mm. thus this proves that silver nanoparticles (SNPs) synthesized by the raw mango peels is potent against the bacteria E. coli.