Pyrazinamide (PYZ), is a first line drug used in treating tuberculosis. it is largely bacteriostatic but sometimes bactericidal on actively replicating tuberculosis bacteria. It is the least understood TB drugs. A rapid, sensitive, simple and cost-effective high performance liquid chromatographic method for the determination of PYZ by UV detection in rabbit plasma which includes extraction process using protein precipitation using a mixture of heptane-1-sulphonoic acid solution with water and acetonitrile in a ratio of 50:50 v/v. PYZ was eluted under isocratic mode using a Luna C18 column. A mixture of heptane-1-sulphonoic acid solution with water and acetonitrile in a ratio of 50:50 v/v was proved to be the most suitable of all the combinations since the chromatography peaks obtained were well defined and resolved and free from tailing. A mobile phase flow rate of 1.0 mL / min. was found to be suitable in the study range of 0.5-1.5 mL/min with a wavelength of detection at 268 nm. The injection volume was 10 μL. The run time of the method was 12 min. The method exhibited good linearity in the range 100-1500 ng/mL. The correlation coefficient (r2) value was found to be 0.999. Precision study showed the % CV value less than 2% in all selected concentrations. The lower limit of quantification (LLOQ) was found to be 100 ng/mL. While, accuracy of LLOQ was found to be 99.9% and %RSD was 0.04%, while the intra- and inter-day accuracies were around accuracies were 98-102% respectively. A rapid, sensitive, simple and cost-effective method for the estimation of pyrazinamide in rabbit plasma was developed and validated according ICH guidelines.
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2022: 6.012
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