Background and objectives: Panton-Valentine leukocidin (LukS-PV and lukF- PV) is a cytotoxin that causes leukocyte destruction and tissue necrosis. PVL-carrying Staphylococcus aureus strains are more virulent and highly transmissible strains than PVL-negative Staphylococcus aureus. To date, PVL has become the most essential and significant virulence factor of community-acquired (CA) S.aureus. This study was conducted to determine the prevalence of Panton-Valentine leukocidin genes in identified CA-MRSA and HA-MRSA isolated from various clinical samples in a tertiary care hospital.
Methods: A prospective cross-sectional study was conducted in a tertiary care hospital at RIMS, Imphal. The study was conducted over a period of three years, from October 2018 to September 2021. The isolates were obtained from various clinical samples such as blood, pus, wound swabs, aspirate, urine, and sputum. PCR amplification of Mec A and PVL genes was performed to check the prevalence among the identified CA-MRSA and HA-MRSA.
Results: 348 Methicillin-resistant Staphylococcus aureus samples were analyzed for Mec A and PVL genes. All 348 samples show positive for Mec A (100%).306 (87.93%) were positive for PVL genes. All 348 MRSA was further identified as HA-MRSA 124 (35.63%) and CA-MRSA 224(64.36%). The PVL-positive rate was high in CA-MRSA 201(89.73%) compared to HA-MRSA 105(84.67%).
Conclusion: Antimicrobial resistance is a major global health concern and of MRSA is a serious threat. PVL gene with added virulence further worsens the clinical outcome among infected patients. Hence the knowledge of its prevalence adds an insight among the infection control practitioners to adhere to effective prevention control.
Impact Factor
2022: 6.012
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