Environmental pollution occurs when the environmental degradation crosses limit so that. It becomes lethal to living organisms. Pollution of water bodies forces them to acclimatize to various factors thus imposing a considerable amount of stress on their lives Assay of Superoxide dismutase (SOD), Assay of Catalase (CAT), Estimation of Reduced glutathione (GSH), Estimation of Glutathione peroxidase (GPX), Acetylcholine (ACh) and acetylcholinesterase activity (AChE),Serum glutamate oxaloacetate transaminase (SGOT), Serum glutamate pyruvate transaminase (SGPT) Liver and Kidney when a freshwater fish Cyprinus carpio exposed to lead acetate concentration as compared to the control group. Solanam virginianum act as alter the acidic and alkaline phosphatase activity in the studied organs three groups of newly hatched spotted Cyprinus carpio were held at three different temperatures in order to determine relationships between metabolic, digestive and growth response in rapidly developing larvae. Reduced glutathione showed a positive compensation (higher activity at a lower temperature) whereas glycolytic enzymes (pyruvate kinase and Lactate dehydrogenase) and aspartate aminotransferase (AST) showed a negative compensation (lower activity at a lower temperature).Hence, the results from present investigations may be useful in the assessment of environmental stress in the aquatic ecosystem.