Antimicrobial susceptibility of soil bacteria isolated from rusting metal dump site, municipal waste dump site and mechanic/engine oil contaminated soil samples in port harcourt

Author: 
Catherine N.Stanley., Kenneth M. Ezealisiji and Eunice N. Chukwuemeka-Eze

High rate of both adult and child mortality from waste related diseases have increased tremendously over the past five decades. Soil samples were taken from three different waste dump sites in Port Harcourt and analysed for the presence of viable bacteria as follows: rustingmetal contaminated soil, municipal waste dump soil and mechanic workshop/engine oil contaminated soil respectively. The pH, temperature and colour of samples were recorded. Bacterial count and antimicrobial susceptibility tests for isolated organisms were carried out using standard microbiological procedures. The bacteria isolated from waste dumping sites included Escherichia coli, Staphylococcus aureus, Klebsiella pneumonia, Bacillus subtilis, Enterobacter amnigenus, Enterobacter gerognes and Salmonella bongery. Bacterial count of microorganisms from the dumpsite ranged between 4.24 x 104 and 5.62 x 104 cfu/g. Soils from the dump sites had a temperature range of 26oC to 27oC and pH range of 6.8 –7.4 respectively. Silver nanoparticles synthesized in the laboratory had the most effective antimicrobial activity against the isolates followed by Erythromycin, Gentamicin and the Cephalosporins. Isolates showed high resistance to Augmentin® and Cloxacillin. Ahigh prevalence of enterobacteria was observed probably due to the dumping of faecal matter contaminated waste at the municipal waste dump.

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DOI: 
http://dx.doi.org/10.24327/ijcar.2018.10347.1747
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