The crude extracts (aqueous and ethanol) of the leaves of Gardenia latifolia were studied for their antibacterial activity by Disc diffusion method against bacterial strains Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Salmonella typhi, Klebsiella pneumoniae, Shigella flexneri, Pseudomonas fluroscence and fungal strains Candia albicans and Aspergillus niger. It was observed that, ethanol extract showed the significant antibacterial activity against Staphylococcus aureus followed by other microorganisms. The MIC values were obtained by serial dilution techniques.