The effect of Syzygium aromaticum L. essential oil extract on soybean seed-borne fungi was investigated. The essential oil was characterized thorough phytochemical analysis, thin layer chromatogram (TLC), column chromatography and gas chromatography-mass spectrometry (GC-MS). Eugenol was found to be the major compound present in the extract. Soybean seed-borne fungi such as Aspergillusflavus, Cladosporium cladosporioides, Fusarium oxysporum and Macrophomina phaseolina were isolated and identified with served as a test fungi. The maximum inhibition was observed in F. oxysporum and M. phaseolina (100%) followed by A. flavus (87%) and C.cladosporioides (84%). The result suggests that the eugenol extracted from Syzygium aromaticum is capable of rendering antifungal efficacy and hence, has a great potential in reducing soybean seed-borne fungal diseases with mycotoxin contamination.