Curcuma is the largest genus in the Zingiberaceae and common use as food, spices, herbal medicines, dyes, and beauty treatments. In this study, the antibacterial properties of Curcuma longa and Curcuma aeruginosa- two popular Curcuma species collected from Lao PDR were investigated using agar well diffusion method. Plant crude extract and its fractional n-hexane, methanol, ethylacetate and dichloromethane from C. aeruginosa showed strong inhibitory activity against S. aureus and P. aeruginosa with the MIC values ranging from 53,3 to 180µg/mL. Three of four fractional extracts from C. longa (methanol, ethylacetate and dichloromethane) suppressed the development of S. aureus while only n-hexane and dichloromethane fractions express similar properties. The MICs values of C. longa were in the range of 110 to 2 15µg/mL.