‘Antibacterial activity of Marine Actinomycetes against dental caries bacteria Streptococcus mutans.’ is a work to recognize the Marine Actinomycetes is capable for control dental caries bacteria Streptococcus mutans. In this study there are 82 Marine Actinomycetes strains isolated from four marine sediment samples from Vizhinjam coastal region, Thiruvananthapuram district in Kerala. The agar overlay method and well and disc diffusion methods were used to check the antibacterial activity of isolated marine actinomycetes against dental caries bacteria Streptococcus mutans. 52 marine actinomycetes isolates showed antibacterial activity in primary screening agar overlay method and 6 marine actinomycetes showed significant antibacterial property in secondary screening well and disc diffusion methods against Streptococcus mutans. The result evidence that the isolated marine actinomycetes capable to producing some secondary metabolites with antibacterial properties against dental caries bacteria Streptococcus mutans.