Analysis of impurities by x-ray diffraction after purification process of the bacterial polysaccharide streptococcus agalactiae

Through this study it is clear that each purification process of the bacterial polysaccharide is different, obtaining different results of X-ray diffraction patterns. The MRI (magnetic resonance imaging) methodology for the characterization of polysaccharides during the production of vaccines is currently used and there are few studies conducted by X-ray diffraction for molecular characterization in the vaccine area. The use of X-ray diffraction can contribute to a better description of the processes and thus the reduction / removal of impurities caused by the decomposition of the product or the manufacturing process, improving the discussion of the potential impact on quality, safety and efficacy.
Polysaccharides are high molecular weight polymers with repeating units composed of glucose, galactose, N-acetylglucosamine and sialic acid. Some bacterial capsular polysaccharides possess virulence factors and are responsible for pathogenicity. The bacterial strain of Streptococcus agalactiae ATCC 12386, corresponding to serotype Ia, was cultivated and four different polysaccharide purification processes with different reagents were performed. X-ray diffraction was carried out to evaluate the identification of molecules of the polysaccharide. Through this study, could be observed that, each purification process of the bacterial polysaccharide is different, obtaining different results of X-ray diffraction patterns. The magnetic resonance imaging methodology for the characterization of polysaccharides during the production of vaccines is currently used, wherein it is proved that the X-ray diffraction could be a useful tool as a complementary molecular characterization methodology for identifying impurities in the purification process of a biological product.The present article focuses on the possibility of using the X - ray diffraction technique as an analytical methodology to evaluate the purification process, free of impurities. It can be useful as methodology of process route analysis and identification of contaminants. At work, we are not characterizing the monosaccharides and their composition, but it would be interesting more studies to compare the purity of materials.