An 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide mediated nanohydrogels formation via 4-hydroxyl phenyl acetamide conjugated chitosan and tyramine functionalized gelatin

An enzymatically fabrication of phenolic chitosan-gelatin (PC-PG) bases hydrogels as encapsulating biphasic calcium phosphate nanoparticles (BCPNPs) and injectable nanocomposite hydrogels (INhydrogel) have much attention in biomedical applications for regenerating hard-soft tissues engineering. Enzymatic peroxidase catalyzed reaction which may proceeded by 4-hydroxyl phenyl acetamide conjugated polysaccharide (chitosan) and tyramine functionalized proteinous gelatin in presence of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide with BCPNPs. The formation of BCPNPs-encapsulated PG-PC and INhydrogel enhanced biomineralization in a phosphate buffer saline (PBS) solution at 37℃ about pH 7.4 about 28 days incubation period on the composite surface in the simulated biofluid which have significant in proliferation of bone marrow mesenchymal stem cells for typical bone cell regeneration in specific tissue. The synthesized nanohydrogels having binding domains due to free functional groups of gelatin and chitosan binding to –OH group in BCP resulting crosslinking density of IN hydrogels for enhanced cell attachment which indicating supporting effect on cell growth or migration of bioactive components or replacement of damage tissue cells.